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Description
In this study, gk gene was disrupted from xylitol dehydrogenase gene (XYL2) knockout C. tropicalis strain BSXDH-3. The resulting strain was incapable to grow on glycerol. The cells growth on glycerol was resumed by co-expression of gcy1, 2 and 3 genes from Scheffersomyces stipitis under the control of C. tropicalis glyceraldehydes-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "IA-3", than in BSXDH-3. The pentose phosphate pathway (PPP) is a major basis of NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes, which respectively encode glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. This is the first report of increased metabolic flux toward glycerol metabolic and PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production.
Spécifications
Parties prenantes
- Auteur(s) :
- Editeur:
Contenu
- Nombre de pages :
- 108
- Langue:
- Anglais
Caractéristiques
- EAN:
- 9783847335016
- Date de parution :
- 03-01-12
- Format:
- Livre broché
- Format numérique:
- Trade paperback (VS)
- Dimensions :
- 152 mm x 229 mm
- Poids :
- 167 g
