Description

Electron Microscopy in Microbiology provides detailed descriptions of electron microscopic techniques relevant to all fields of microbiological research. In numerous protocols, standard techniques required for the structural characterization of unicellular organisms (suspended or fixed in microbial biofilms) and subcellular components (enzymes, nucleic acids, inclusion bodies, cell appendages etc) are described. Particular attention is given to techniques that provide information on the structure and compartmentation of biological macromolecules within the cell. These techniques include epitope mapping and localization of proteins by antibodies and other marker systems (DNA/RNA probes, lectins etc).