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- Toujours un magasin près de chez vous
Low-Cost Methods for Molecular Characterization of Mutant Plants
Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Bradley J Till, Joanna Jankowicz-Cieslak, Owen A Huynh, Mayada M Beshir, Robert G Laport, Bernhard J Hofinger
Livre broché | Anglais
52,95 €
+ 105 points
Format
Description
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Spécifications
Parties prenantes
- Auteur(s) :
- Editeur:
Contenu
- Nombre de pages :
- 38
- Langue:
- Anglais
Caractéristiques
- EAN:
- 9783319386676
- Date de parution :
- 17-10-16
- Format:
- Livre broché
- Format numérique:
- Trade paperback (VS)
- Dimensions :
- 156 mm x 234 mm
- Poids :
- 81 g
