The generation of transgenic mammals, mostly rodents, has become one of the most important approaches in biological and biomedical research, and is of utmost importance in the generation of genetically improved livestock. Edited by respected scholars at the California Institute of Technology, this book provides step-by-step protocols of this important technique. The manual covers not only mice and other rodents, it also describes methods successfully used in cattle, sheep and pigs, making it extremely useful for anyone working on mammalian transgenesis.